Construct: shRNA TRCN0000272118

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001110250.2-498s21c1
DNA Barcode:
TGAAGAAGTAGTTGGAGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm14819 (100039585)
Gene Description:
predicted gene 14819
Transcript:
RefSeq NM_001110250.2 (CURRENT)
Match location:
Position 498 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272118)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGAAGTAGTTGGAGATAC
Hairpin Sequence:
5'-CCGG-TGAAGAAGTAGTTGGAGATAC-CTCGAG-GTATCTCCAACTACTTCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGAAGTAGTTGGAGATACCTCGAGGTATCTCCAACTACTTCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGAAGTAGTTGGAGATACCTCGAGGTATCTCCAACTACTTCTTCA-3'

Other clones with same target sequence:

(none)