Construct: shRNA TRCN0000272223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001109969.2-369s21c1
DNA Barcode:
ACGTTCTGTAGAGACACCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm10058 (100039240)
Gene Description:
predicted gene 10058
Transcript:
RefSeq NM_001109969.2 (CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTTCTGTAGAGACACCAAT
Hairpin Sequence:
5'-CCGG-ACGTTCTGTAGAGACACCAAT-CTCGAG-ATTGGTGTCTCTACAGAACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTTCTGTAGAGACACCAATCTCGAGATTGGTGTCTCTACAGAACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTTCTGTAGAGACACCAATCTCGAGATTGGTGTCTCTACAGAACGT-3'

Other clones with same target sequence:

(none)