Construct: shRNA TRCN0000272240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001102678.1-216s21c1
DNA Barcode:
TTGACTATGATTCAGATGAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm10096 (100039377)
Gene Description:
predicted gene 10096
Transcript:
RefSeq NM_001102678.1 (NON-CURRENT)
Match location:
Position 216 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACTATGATTCAGATGAAG
Hairpin Sequence:
5'-CCGG-TTGACTATGATTCAGATGAAG-CTCGAG-CTTCATCTGAATCATAGTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACTATGATTCAGATGAAGCTCGAGCTTCATCTGAATCATAGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACTATGATTCAGATGAAGCTCGAGCTTCATCTGAATCATAGTCAA-3'

Other clones with same target sequence:

(none)