Construct: shRNA TRCN0000272266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001134675.1-505s21c1
DNA Barcode:
ACCTAAGACAGACTATGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mup7 (100041658)
Gene Description:
major urinary protein 7
Transcript:
RefSeq NM_001134675.1 (CURRENT)
Match location:
Position 505 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTAAGACAGACTATGATAA
Hairpin Sequence:
5'-CCGG-ACCTAAGACAGACTATGATAA-CTCGAG-TTATCATAGTCTGTCTTAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTAAGACAGACTATGATAACTCGAGTTATCATAGTCTGTCTTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTAAGACAGACTATGATAACTCGAGTTATCATAGTCTGTCTTAGGT-3'

Other clones with same target sequence:

(none)