Construct: shRNA TRCN0000272305

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015151.2-4346s21c1
DNA Barcode:
CACCGGGTACTACACCGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DIP2A (23181)
Gene Description:
disco interacting protein 2 homolog A
Transcript:
RefSeq NM_015151.2 (NON-CURRENT)
Match location:
Position 4346 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272305)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCGGGTACTACACCGTTTA
Hairpin Sequence:
5'-CCGG-CACCGGGTACTACACCGTTTA-CTCGAG-TAAACGGTGTAGTACCCGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCGGGTACTACACCGTTTACTCGAGTAAACGGTGTAGTACCCGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCGGGTACTACACCGTTTACTCGAGTAAACGGTGTAGTACCCGGTG-3'

Other clones with same target sequence:

(none)