Construct: shRNA TRCN0000272312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014990.1-6010s21c1
DNA Barcode:
CTCTACAGTAGAGGTAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGAPA1 (253959)
Gene Description:
Ral GTPase activating protein catalytic alpha subunit 1
Transcript:
RefSeq NM_014990.1 (NON-CURRENT)
Match location:
Position 6010 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTACAGTAGAGGTAATATT
Hairpin Sequence:
5'-CCGG-CTCTACAGTAGAGGTAATATT-CTCGAG-AATATTACCTCTACTGTAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTACAGTAGAGGTAATATTCTCGAGAATATTACCTCTACTGTAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTACAGTAGAGGTAATATTCTCGAGAATATTACCTCTACTGTAGAG-3'

Other clones with same target sequence:

(none)