Construct: shRNA TRCN0000272411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001130446.2-733s21c1
DNA Barcode:
TCAAGTCATTAACGAATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CC2D2B (387707)
Gene Description:
coiled-coil and C2 domain containing 2B
Transcript:
RefSeq NM_001130446.2 (CURRENT)
Match location:
Position 733 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGTCATTAACGAATTATG
Hairpin Sequence:
5'-CCGG-TCAAGTCATTAACGAATTATG-CTCGAG-CATAATTCGTTAATGACTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGTCATTAACGAATTATGCTCGAGCATAATTCGTTAATGACTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGTCATTAACGAATTATGCTCGAGCATAATTCGTTAATGACTTGA-3'

Other clones with same target sequence:

(none)