Construct: shRNA TRCN0000272448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003338.3-370s21c1
DNA Barcode:
CTTCTTTCTCACTGTACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2D1 (7321)
Gene Description:
ubiquitin conjugating enzyme E2 D1
Transcript:
RefSeq NM_003338.3 (NON-CURRENT)
Match location:
Position 370 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTTTCTCACTGTACATTT
Hairpin Sequence:
5'-CCGG-CTTCTTTCTCACTGTACATTT-CTCGAG-AAATGTACAGTGAGAAAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTTTCTCACTGTACATTTCTCGAGAAATGTACAGTGAGAAAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTTTCTCACTGTACATTTCTCGAGAAATGTACAGTGAGAAAGAAG-3'

Other clones with same target sequence:

  1. TRCN0000003381