Construct: shRNA TRCN0000272470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001142308.1-923s21c1
DNA Barcode:
TGCTCTACAGACGAGTGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC100128641 (100128641)
Gene Description:
similar to hCG1641533
Transcript:
RefSeq NM_001142308.1 (NON-CURRENT)
Match location:
Position 923 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTCTACAGACGAGTGTATA
Hairpin Sequence:
5'-CCGG-TGCTCTACAGACGAGTGTATA-CTCGAG-TATACACTCGTCTGTAGAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTCTACAGACGAGTGTATACTCGAGTATACACTCGTCTGTAGAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTCTACAGACGAGTGTATACTCGAGTATACACTCGTCTGTAGAGCA-3'

Other clones with same target sequence:

(none)