Construct: shRNA TRCN0000272499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015983.2-494s21c1
DNA Barcode:
CTAGCAAGAGAGTGGACACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2D4 (51619)
Gene Description:
ubiquitin conjugating enzyme E2 D4 (putative)
Transcript:
RefSeq NM_015983.2 (NON-CURRENT)
Match location:
Position 494 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGCAAGAGAGTGGACACAA
Hairpin Sequence:
5'-CCGG-CTAGCAAGAGAGTGGACACAA-CTCGAG-TTGTGTCCACTCTCTTGCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGCAAGAGAGTGGACACAACTCGAGTTGTGTCCACTCTCTTGCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGCAAGAGAGTGGACACAACTCGAGTTGTGTCCACTCTCTTGCTAG-3'

Other clones with same target sequence:

(none)