Construct: shRNA TRCN0000272506

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002815.2-1116s21c1
DNA Barcode:
CCGACGTGGAAAGGAAATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMD11 (5717)
Gene Description:
proteasome 26S subunit, non-ATPase 11
Transcript:
RefSeq NM_002815.2 (NON-CURRENT)
Match location:
Position 1116 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272506)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACGTGGAAAGGAAATTAT
Hairpin Sequence:
5'-CCGG-CCGACGTGGAAAGGAAATTAT-CTCGAG-ATAATTTCCTTTCCACGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACGTGGAAAGGAAATTATCTCGAGATAATTTCCTTTCCACGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACGTGGAAAGGAAATTATCTCGAGATAATTTCCTTTCCACGTCGG-3'

Other clones with same target sequence:

  1. TRCN0000003950