Construct: shRNA TRCN0000272512

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004760.2-1488s21c1
DNA Barcode:
TGGATAACCAGTATCACTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK17A (9263)
Gene Description:
serine/threonine kinase 17a
Transcript:
RefSeq NM_004760.2 (NON-CURRENT)
Match location:
Position 1488 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272512)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATAACCAGTATCACTTAC
Hairpin Sequence:
5'-CCGG-TGGATAACCAGTATCACTTAC-CTCGAG-GTAAGTGATACTGGTTATCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATAACCAGTATCACTTACCTCGAGGTAAGTGATACTGGTTATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATAACCAGTATCACTTACCTCGAGGTAAGTGATACTGGTTATCCA-3'

Other clones with same target sequence:

(none)