Construct: shRNA TRCN0000272516
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000094.3-1178s21c1
- DNA Barcode:
- GTGACCGTGAGCACCCTATTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- COL7A1 (1294)
- Gene Description:
- collagen type VII alpha 1 chain
- Transcript:
- RefSeq NM_000094.3 (CURRENT)
- Match location:
- Position 1178 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000272516)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTGACCGTGAGCACCCTATTT
- Hairpin Sequence:
-
5'-CCGG-GTGACCGTGAGCACCCTATTT-CTCGAG-AAATAGGGTGCTCACGGTCAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTGACCGTGAGCACCCTATTTCTCGAGAAATAGGGTGCTCACGGTCACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTGACCGTGAGCACCCTATTTCTCGAGAAATAGGGTGCTCACGGTCAC-3'
Other clones with same target sequence:
(none)
