Construct: shRNA TRCN0000272545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080391.2-1438s21c1
DNA Barcode:
ATACCGACCTAAGATGCGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTP4A2 (8073)
Gene Description:
protein tyrosine phosphatase 4A2
Transcript:
RefSeq NM_080391.2 (NON-CURRENT)
Match location:
Position 1438 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATACCGACCTAAGATGCGATT
Hairpin Sequence:
5'-CCGG-ATACCGACCTAAGATGCGATT-CTCGAG-AATCGCATCTTAGGTCGGTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATACCGACCTAAGATGCGATTCTCGAGAATCGCATCTTAGGTCGGTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATACCGACCTAAGATGCGATTCTCGAGAATCGCATCTTAGGTCGGTAT-3'

Other clones with same target sequence:

  1. TRCN0000002926