Construct: shRNA TRCN0000272656

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003333.3-443s21c1
DNA Barcode:
CACCAACAACCTGCGTCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBA52 (7311)
Gene Description:
ubiquitin A-52 residue ribosomal protein fusion product 1
Transcript:
RefSeq NM_003333.3 (NON-CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272656)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAACAACCTGCGTCCCAA
Hairpin Sequence:
5'-CCGG-CACCAACAACCTGCGTCCCAA-CTCGAG-TTGGGACGCAGGTTGTTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAACAACCTGCGTCCCAACTCGAGTTGGGACGCAGGTTGTTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAACAACCTGCGTCCCAACTCGAGTTGGGACGCAGGTTGTTGGTG-3'

Other clones with same target sequence:

  1. TRCN0000010841