Construct: shRNA TRCN0000272689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207111.2-2469s21c1
DNA Barcode:
GTCGAGTTTCTATTAATGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF216 (54476)
Gene Description:
ring finger protein 216
Transcript:
RefSeq NM_207111.2 (NON-CURRENT)
Match location:
Position 2469 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGAGTTTCTATTAATGGAT
Hairpin Sequence:
5'-CCGG-GTCGAGTTTCTATTAATGGAT-CTCGAG-ATCCATTAATAGAAACTCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGAGTTTCTATTAATGGATCTCGAGATCCATTAATAGAAACTCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGAGTTTCTATTAATGGATCTCGAGATCCATTAATAGAAACTCGAC-3'

Other clones with same target sequence:

  1. TRCN0000010788