Construct: shRNA TRCN0000272724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018984.2-1101s21c1
DNA Barcode:
TTACCAGAGAAATCGATAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SSH1 (54434)
Gene Description:
slingshot protein phosphatase 1
Transcript:
RefSeq NM_018984.2 (NON-CURRENT)
Match location:
Position 1101 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACCAGAGAAATCGATAATT
Hairpin Sequence:
5'-CCGG-TTACCAGAGAAATCGATAATT-CTCGAG-AATTATCGATTTCTCTGGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACCAGAGAAATCGATAATTCTCGAGAATTATCGATTTCTCTGGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACCAGAGAAATCGATAATTCTCGAGAATTATCGATTTCTCTGGTAA-3'

Other clones with same target sequence:

(none)