Construct: shRNA TRCN0000272735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003089.4-1850s21c1
DNA Barcode:
CCAAGGGTAGGTGTCTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNRNP70 (6625)
Gene Description:
small nuclear ribonucleoprotein U1 subunit 70
Transcript:
RefSeq NM_003089.4 (NON-CURRENT)
Match location:
Position 1850 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGGTAGGTGTCTCATTT
Hairpin Sequence:
5'-CCGG-CCAAGGGTAGGTGTCTCATTT-CTCGAG-AAATGAGACACCTACCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGGTAGGTGTCTCATTTCTCGAGAAATGAGACACCTACCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGGTAGGTGTCTCATTTCTCGAGAAATGAGACACCTACCCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000000011