Construct: shRNA TRCN0000272772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005802.2-3608s21c1
DNA Barcode:
CCATACGGTATTGACATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOPORS (10210)
Gene Description:
TOP1 binding arginine/serine rich protein
Transcript:
RefSeq NM_005802.2 (NON-CURRENT)
Match location:
Position 3608 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATACGGTATTGACATATTT
Hairpin Sequence:
5'-CCGG-CCATACGGTATTGACATATTT-CTCGAG-AAATATGTCAATACCGTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATACGGTATTGACATATTTCTCGAGAAATATGTCAATACCGTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATACGGTATTGACATATTTCTCGAGAAATATGTCAATACCGTATGG-3'

Other clones with same target sequence:

(none)