Construct: shRNA TRCN0000272784

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014003.3-1990s21c1
DNA Barcode:
CAGAGACAACAGCATCGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHX38 (9785)
Gene Description:
DEAH-box helicase 38
Transcript:
RefSeq NM_014003.3 (NON-CURRENT)
Match location:
Position 1990 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272784)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGACAACAGCATCGTGAT
Hairpin Sequence:
5'-CCGG-CAGAGACAACAGCATCGTGAT-CTCGAG-ATCACGATGCTGTTGTCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGACAACAGCATCGTGATCTCGAGATCACGATGCTGTTGTCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGACAACAGCATCGTGATCTCGAGATCACGATGCTGTTGTCTCTG-3'

Other clones with same target sequence:

  1. TRCN0000001174