Construct: shRNA TRCN0000272808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003800.3-1363s21c1
DNA Barcode:
GCAGTGTATAGAACGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNGTT (8732)
Gene Description:
RNA guanylyltransferase and 5'-phosphatase
Transcript:
RefSeq NM_003800.3 (NON-CURRENT)
Match location:
Position 1363 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGTATAGAACGAGAAAT
Hairpin Sequence:
5'-CCGG-GCAGTGTATAGAACGAGAAAT-CTCGAG-ATTTCTCGTTCTATACACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGTATAGAACGAGAAATCTCGAGATTTCTCGTTCTATACACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGTATAGAACGAGAAATCTCGAGATTTCTCGTTCTATACACTGC-3'

Other clones with same target sequence:

  1. TRCN0000002589