Construct: shRNA TRCN0000272826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005802.2-2218s21c1
DNA Barcode:
GCAGAAATAGAGATCGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOPORS (10210)
Gene Description:
TOP1 binding arginine/serine rich protein
Transcript:
RefSeq NM_005802.2 (NON-CURRENT)
Match location:
Position 2218 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAATAGAGATCGTTATT
Hairpin Sequence:
5'-CCGG-GCAGAAATAGAGATCGTTATT-CTCGAG-AATAACGATCTCTATTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAATAGAGATCGTTATTCTCGAGAATAACGATCTCTATTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAATAGAGATCGTTATTCTCGAGAATAACGATCTCTATTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000007517