Construct: shRNA TRCN0000272892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006491.2-790s21c1
DNA Barcode:
ACCAAGTCCTCTCCATCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOVA1 (4857)
Gene Description:
NOVA alternative splicing regulator 1
Transcript:
RefSeq NM_006491.2 (CURRENT)
Match location:
Position 790 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAAGTCCTCTCCATCTGAT
Hairpin Sequence:
5'-CCGG-ACCAAGTCCTCTCCATCTGAT-CTCGAG-ATCAGATGGAGAGGACTTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAAGTCCTCTCCATCTGATCTCGAGATCAGATGGAGAGGACTTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAAGTCCTCTCCATCTGATCTCGAGATCAGATGGAGAGGACTTGGT-3'

Other clones with same target sequence:

  1. TRCN0000001091