Construct: shRNA TRCN0000272909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022066.3-1981s21c1
DNA Barcode:
ATGTGAGTGTTTACGACATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2O (63893)
Gene Description:
ubiquitin conjugating enzyme E2 O
Transcript:
RefSeq NM_022066.3 (NON-CURRENT)
Match location:
Position 1981 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTGAGTGTTTACGACATTG
Hairpin Sequence:
5'-CCGG-ATGTGAGTGTTTACGACATTG-CTCGAG-CAATGTCGTAAACACTCACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTGAGTGTTTACGACATTGCTCGAGCAATGTCGTAAACACTCACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTGAGTGTTTACGACATTGCTCGAGCAATGTCGTAAACACTCACAT-3'

Other clones with same target sequence:

(none)