Construct: shRNA TRCN0000273162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006180.3-2579s21c1
DNA Barcode:
GCACATCAAGCGACATAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTRK2 (4915)
Gene Description:
neurotrophic receptor tyrosine kinase 2
Transcript:
RefSeq NM_006180.3 (NON-CURRENT)
Match location:
Position 2579 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATCAAGCGACATAACAT
Hairpin Sequence:
5'-CCGG-GCACATCAAGCGACATAACAT-CTCGAG-ATGTTATGTCGCTTGATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATCAAGCGACATAACATCTCGAGATGTTATGTCGCTTGATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATCAAGCGACATAACATCTCGAGATGTTATGTCGCTTGATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000002245