Construct: shRNA TRCN0000273169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015508.3-1949s21c1
DNA Barcode:
GAAGGCAAGCTACTCTCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIPARP (25976)
Gene Description:
TCDD inducible poly(ADP-ribose) polymerase
Transcript:
RefSeq NM_015508.3 (NON-CURRENT)
Match location:
Position 1949 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGCAAGCTACTCTCATAA
Hairpin Sequence:
5'-CCGG-GAAGGCAAGCTACTCTCATAA-CTCGAG-TTATGAGAGTAGCTTGCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGCAAGCTACTCTCATAACTCGAGTTATGAGAGTAGCTTGCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGCAAGCTACTCTCATAACTCGAGTTATGAGAGTAGCTTGCCTTC-3'

Other clones with same target sequence:

  1. TRCN0000004462