Construct: shRNA TRCN0000273182

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003021.3-2184s21c1
DNA Barcode:
CGTGTATCGTAGGTAGCAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGTA (6449)
Gene Description:
small glutamine rich tetratricopeptide repeat containing alpha
Transcript:
RefSeq NM_003021.3 (NON-CURRENT)
Match location:
Position 2184 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273182)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTATCGTAGGTAGCAGTA
Hairpin Sequence:
5'-CCGG-CGTGTATCGTAGGTAGCAGTA-CTCGAG-TACTGCTACCTACGATACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTATCGTAGGTAGCAGTACTCGAGTACTGCTACCTACGATACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTATCGTAGGTAGCAGTACTCGAGTACTGCTACCTACGATACACG-3'

Other clones with same target sequence:

  1. TRCN0000007191