Construct: shRNA TRCN0000273210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004238.1-3204s21c1
DNA Barcode:
CCACTACTCAGTCACCTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIP12 (9320)
Gene Description:
thyroid hormone receptor interactor 12
Transcript:
RefSeq NM_004238.1 (NON-CURRENT)
Match location:
Position 3204 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTACTCAGTCACCTAAAT
Hairpin Sequence:
5'-CCGG-CCACTACTCAGTCACCTAAAT-CTCGAG-ATTTAGGTGACTGAGTAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTACTCAGTCACCTAAATCTCGAGATTTAGGTGACTGAGTAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTACTCAGTCACCTAAATCTCGAGATTTAGGTGACTGAGTAGTGG-3'

Other clones with same target sequence:

(none)