Construct: shRNA TRCN0000273276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012426.3-3185s21c1
DNA Barcode:
CTATCGGACATAGGGTAATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SF3B3 (23450)
Gene Description:
splicing factor 3b subunit 3
Transcript:
RefSeq NM_012426.3 (NON-CURRENT)
Match location:
Position 3185 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATCGGACATAGGGTAATTG
Hairpin Sequence:
5'-CCGG-CTATCGGACATAGGGTAATTG-CTCGAG-CAATTACCCTATGTCCGATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATCGGACATAGGGTAATTGCTCGAGCAATTACCCTATGTCCGATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATCGGACATAGGGTAATTGCTCGAGCAATTACCCTATGTCCGATAG-3'

Other clones with same target sequence:

(none)