Construct: shRNA TRCN0000273284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001396.3-118s21c1
DNA Barcode:
GTTCGGCTTGCACCGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYRK1A (1859)
Gene Description:
dual specificity tyrosine phosphorylation regulated kinase 1A
Transcript:
RefSeq NM_001396.3 (NON-CURRENT)
Match location:
Position 118 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGGCTTGCACCGTCATTT
Hairpin Sequence:
5'-CCGG-GTTCGGCTTGCACCGTCATTT-CTCGAG-AAATGACGGTGCAAGCCGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGGCTTGCACCGTCATTTCTCGAGAAATGACGGTGCAAGCCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGGCTTGCACCGTCATTTCTCGAGAAATGACGGTGCAAGCCGAAC-3'

Other clones with same target sequence:

(none)