Construct: shRNA TRCN0000273326

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002845.3-1500s21c1
DNA Barcode:
GTCGATTCCACGAGCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRM (5797)
Gene Description:
protein tyrosine phosphatase receptor type M
Transcript:
RefSeq NM_002845.3 (CURRENT)
Match location:
Position 1500 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273326)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGATTCCACGAGCTATAAA
Hairpin Sequence:
5'-CCGG-GTCGATTCCACGAGCTATAAA-CTCGAG-TTTATAGCTCGTGGAATCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGATTCCACGAGCTATAAACTCGAGTTTATAGCTCGTGGAATCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGATTCCACGAGCTATAAACTCGAGTTTATAGCTCGTGGAATCGAC-3'

Other clones with same target sequence:

(none)