Construct: shRNA TRCN0000273338

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001731.2-413s21c1
DNA Barcode:
CGCCGTGTCCTTCATCTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BTG1 (694)
Gene Description:
BTG anti-proliferation factor 1
Transcript:
RefSeq NM_001731.2 (NON-CURRENT)
Match location:
Position 413 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273338)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCGTGTCCTTCATCTCCAA
Hairpin Sequence:
5'-CCGG-CGCCGTGTCCTTCATCTCCAA-CTCGAG-TTGGAGATGAAGGACACGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCGTGTCCTTCATCTCCAACTCGAGTTGGAGATGAAGGACACGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCGTGTCCTTCATCTCCAACTCGAGTTGGAGATGAAGGACACGGCG-3'

Other clones with same target sequence:

  1. TRCN0000003824