Construct: shRNA TRCN0000273350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001396.3-472s21c1
DNA Barcode:
CGGAAGGTTTACAATGATGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYRK1A (1859)
Gene Description:
dual specificity tyrosine phosphorylation regulated kinase 1A
Transcript:
RefSeq NM_001396.3 (NON-CURRENT)
Match location:
Position 472 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGGTTTACAATGATGGT
Hairpin Sequence:
5'-CCGG-CGGAAGGTTTACAATGATGGT-CTCGAG-ACCATCATTGTAAACCTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGGTTTACAATGATGGTCTCGAGACCATCATTGTAAACCTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGGTTTACAATGATGGTCTCGAGACCATCATTGTAAACCTTCCG-3'

Other clones with same target sequence:

  1. TRCN0000000526
  2. TRCN0000010613