Construct: shRNA TRCN0000273400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031684.2-716s21c1
DNA Barcode:
CCTCGACGATCAAGATCTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRSF7 (6432)
Gene Description:
serine and arginine rich splicing factor 7
Transcript:
RefSeq NM_001031684.2 (NON-CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGACGATCAAGATCTATC
Hairpin Sequence:
5'-CCGG-CCTCGACGATCAAGATCTATC-CTCGAG-GATAGATCTTGATCGTCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGACGATCAAGATCTATCCTCGAGGATAGATCTTGATCGTCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGACGATCAAGATCTATCCTCGAGGATAGATCTTGATCGTCGAGG-3'

Other clones with same target sequence:

(none)