Construct: shRNA TRCN0000273496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018842.3-724s21c1
DNA Barcode:
CGTCAGAGTGAAATCCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAIAP2L1 (55971)
Gene Description:
BAR/IMD domain containing adaptor protein 2 like 1
Transcript:
RefSeq NM_018842.3 (NON-CURRENT)
Match location:
Position 724 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAGAGTGAAATCCAGAAA
Hairpin Sequence:
5'-CCGG-CGTCAGAGTGAAATCCAGAAA-CTCGAG-TTTCTGGATTTCACTCTGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAGAGTGAAATCCAGAAACTCGAGTTTCTGGATTTCACTCTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAGAGTGAAATCCAGAAACTCGAGTTTCTGGATTTCACTCTGACG-3'

Other clones with same target sequence:

  1. TRCN0000005350