Construct: shRNA TRCN0000273530

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001684.3-5184s21c1
DNA Barcode:
CCCTTGATTTAGTTCCAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP2B4 (493)
Gene Description:
ATPase plasma membrane Ca2+ transporting 4
Transcript:
RefSeq NM_001684.3 (NON-CURRENT)
Match location:
Position 5184 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273530)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGATTTAGTTCCAGAAT
Hairpin Sequence:
5'-CCGG-CCCTTGATTTAGTTCCAGAAT-CTCGAG-ATTCTGGAACTAAATCAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGATTTAGTTCCAGAATCTCGAGATTCTGGAACTAAATCAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGATTTAGTTCCAGAATCTCGAGATTCTGGAACTAAATCAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000005781