Construct: shRNA TRCN0000273619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004528.2-284s21c1
DNA Barcode:
GAAGTGTATCCTCCCTTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGST3 (4259)
Gene Description:
microsomal glutathione S-transferase 3
Transcript:
RefSeq NM_004528.2 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGTATCCTCCCTTCTTA
Hairpin Sequence:
5'-CCGG-GAAGTGTATCCTCCCTTCTTA-CTCGAG-TAAGAAGGGAGGATACACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGTATCCTCCCTTCTTACTCGAGTAAGAAGGGAGGATACACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGTATCCTCCCTTCTTACTCGAGTAAGAAGGGAGGATACACTTC-3'

Other clones with same target sequence:

  1. TRCN0000154794