Construct: shRNA TRCN0000273636

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012234.4-937s21c1
DNA Barcode:
ATTTCGCACCCTGACGATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RYBP (23429)
Gene Description:
RING1 and YY1 binding protein
Transcript:
RefSeq NM_012234.4 (NON-CURRENT)
Match location:
Position 937 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273636)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTCGCACCCTGACGATTAC
Hairpin Sequence:
5'-CCGG-ATTTCGCACCCTGACGATTAC-CTCGAG-GTAATCGTCAGGGTGCGAAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTCGCACCCTGACGATTACCTCGAGGTAATCGTCAGGGTGCGAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTCGCACCCTGACGATTACCTCGAGGTAATCGTCAGGGTGCGAAAT-3'

Other clones with same target sequence:

(none)