Construct: shRNA TRCN0000273706
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_053208.3-1489s21c1
- DNA Barcode:
- AGGTGTTCAAGTACCAGTATC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Egln2 (112406)
- Gene Description:
- egl-9 family hypoxia-inducible factor 2
- Transcript:
- RefSeq NM_053208.3 (NON-CURRENT)
- Match location:
- Position 1489 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000273706)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGGTGTTCAAGTACCAGTATC
- Hairpin Sequence:
-
5'-CCGG-AGGTGTTCAAGTACCAGTATC-CTCGAG-GATACTGGTACTTGAACACCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGGTGTTCAAGTACCAGTATCCTCGAGGATACTGGTACTTGAACACCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGGTGTTCAAGTACCAGTATCCTCGAGGATACTGGTACTTGAACACCT-3'
Other clones with same target sequence:
(none)
