Construct: shRNA TRCN0000273729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010905.3-663s21c1
DNA Barcode:
CCCTGATCAGAAAGGCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfia (18027)
Gene Description:
nuclear factor I/A
Transcript:
RefSeq NM_010905.3 (CURRENT)
Match location:
Position 663 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGATCAGAAAGGCAAGAT
Hairpin Sequence:
5'-CCGG-CCCTGATCAGAAAGGCAAGAT-CTCGAG-ATCTTGCCTTTCTGATCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGATCAGAAAGGCAAGATCTCGAGATCTTGCCTTTCTGATCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGATCAGAAAGGCAAGATCTCGAGATCTTGCCTTTCTGATCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000012085