Construct: shRNA TRCN0000273770

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133753.1-2092s21c1
DNA Barcode:
CATACAGGCCTGCTCTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Errfi1 (74155)
Gene Description:
ERBB receptor feedback inhibitor 1
Transcript:
RefSeq NM_133753.1 (CURRENT)
Match location:
Position 2092 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273770)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATACAGGCCTGCTCTATAAA
Hairpin Sequence:
5'-CCGG-CATACAGGCCTGCTCTATAAA-CTCGAG-TTTATAGAGCAGGCCTGTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATACAGGCCTGCTCTATAAACTCGAGTTTATAGAGCAGGCCTGTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATACAGGCCTGCTCTATAAACTCGAGTTTATAGAGCAGGCCTGTATG-3'

Other clones with same target sequence:

(none)