Construct: shRNA TRCN0000273805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014246.1-4988s21c1
DNA Barcode:
CCTGTGTCAACAGGTGGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CELSR1 (9620)
Gene Description:
cadherin EGF LAG seven-pass G-type receptor 1
Transcript:
RefSeq NM_014246.1 (NON-CURRENT)
Match location:
Position 4988 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTCAACAGGTGGAATA
Hairpin Sequence:
5'-CCGG-CCTGTGTCAACAGGTGGAATA-CTCGAG-TATTCCACCTGTTGACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTCAACAGGTGGAATACTCGAGTATTCCACCTGTTGACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTCAACAGGTGGAATACTCGAGTATTCCACCTGTTGACACAGG-3'

Other clones with same target sequence:

  1. TRCN0000008242