Construct: shRNA TRCN0000273868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015236.4-498s21c1
DNA Barcode:
CCGTGTCCAGGAACCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRL3 (23284)
Gene Description:
adhesion G protein-coupled receptor L3
Transcript:
RefSeq NM_015236.4 (NON-CURRENT)
Match location:
Position 498 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGTCCAGGAACCTATAAA
Hairpin Sequence:
5'-CCGG-CCGTGTCCAGGAACCTATAAA-CTCGAG-TTTATAGGTTCCTGGACACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGTCCAGGAACCTATAAACTCGAGTTTATAGGTTCCTGGACACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGTCCAGGAACCTATAAACTCGAGTTTATAGGTTCCTGGACACGG-3'

Other clones with same target sequence:

(none)