Construct: shRNA TRCN0000273875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033141.2-2603s21c1
DNA Barcode:
GCGATGAAATTGTCGTGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K9 (4293)
Gene Description:
mitogen-activated protein kinase kinase kinase 9
Transcript:
RefSeq NM_033141.2 (NON-CURRENT)
Match location:
Position 2603 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATGAAATTGTCGTGTATG
Hairpin Sequence:
5'-CCGG-GCGATGAAATTGTCGTGTATG-CTCGAG-CATACACGACAATTTCATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATGAAATTGTCGTGTATGCTCGAGCATACACGACAATTTCATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATGAAATTGTCGTGTATGCTCGAGCATACACGACAATTTCATCGC-3'

Other clones with same target sequence:

  1. TRCN0000196575