Construct: shRNA TRCN0000273963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004502.3-1124s21c1
DNA Barcode:
GCCCTCTTTAATGCTGTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HOXB7 (3217)
Gene Description:
homeobox B7
Transcript:
RefSeq NM_004502.3 (NON-CURRENT)
Match location:
Position 1124 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000273963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCTTTAATGCTGTCTTT
Hairpin Sequence:
5'-CCGG-GCCCTCTTTAATGCTGTCTTT-CTCGAG-AAAGACAGCATTAAAGAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCTTTAATGCTGTCTTTCTCGAGAAAGACAGCATTAAAGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCTTTAATGCTGTCTTTCTCGAGAAAGACAGCATTAAAGAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000015943