Construct: shRNA TRCN0000274054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005920.2-1110s21c1
DNA Barcode:
CAACAGCCTAAACAAGGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEF2D (4209)
Gene Description:
myocyte enhancer factor 2D
Transcript:
RefSeq NM_005920.2 (NON-CURRENT)
Match location:
Position 1110 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGCCTAAACAAGGTCAT
Hairpin Sequence:
5'-CCGG-CAACAGCCTAAACAAGGTCAT-CTCGAG-ATGACCTTGTTTAGGCTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGCCTAAACAAGGTCATCTCGAGATGACCTTGTTTAGGCTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGCCTAAACAAGGTCATCTCGAGATGACCTTGTTTAGGCTGTTG-3'

Other clones with same target sequence:

  1. TRCN0000015897