Construct: shRNA TRCN0000274128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006166.3-446s21c1
DNA Barcode:
CCAAAGAATGTGTTCAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFYB (4801)
Gene Description:
nuclear transcription factor Y subunit beta
Transcript:
RefSeq NM_006166.3 (NON-CURRENT)
Match location:
Position 446 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGAATGTGTTCAAGAAT
Hairpin Sequence:
5'-CCGG-CCAAAGAATGTGTTCAAGAAT-CTCGAG-ATTCTTGAACACATTCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGAATGTGTTCAAGAATCTCGAGATTCTTGAACACATTCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGAATGTGTTCAAGAATCTCGAGATTCTTGAACACATTCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000014954