Construct: shRNA TRCN0000274145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003079.4-534s21c1
DNA Barcode:
CCGCGTACCTTGCTTACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMARCE1 (6605)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Transcript:
RefSeq NM_003079.4 (NON-CURRENT)
Match location:
Position 534 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCGTACCTTGCTTACATAA
Hairpin Sequence:
5'-CCGG-CCGCGTACCTTGCTTACATAA-CTCGAG-TTATGTAAGCAAGGTACGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCGTACCTTGCTTACATAACTCGAGTTATGTAAGCAAGGTACGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCGTACCTTGCTTACATAACTCGAGTTATGTAAGCAAGGTACGCGG-3'

Other clones with same target sequence:

  1. TRCN0000015779