Construct: shRNA TRCN0000274203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003079.4-725s21c1
DNA Barcode:
CGCCTCATCAGTGAAATTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMARCE1 (6605)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Transcript:
RefSeq NM_003079.4 (NON-CURRENT)
Match location:
Position 725 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTCATCAGTGAAATTCTT
Hairpin Sequence:
5'-CCGG-CGCCTCATCAGTGAAATTCTT-CTCGAG-AAGAATTTCACTGATGAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTCATCAGTGAAATTCTTCTCGAGAAGAATTTCACTGATGAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTCATCAGTGAAATTCTTCTCGAGAAGAATTTCACTGATGAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000015781