Construct: shRNA TRCN0000274216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003200.2-1227s21c1
DNA Barcode:
ACGGCCTGCAGAGTAAGATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCF3 (6929)
Gene Description:
transcription factor 3
Transcript:
RefSeq NM_003200.2 (NON-CURRENT)
Match location:
Position 1227 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGCCTGCAGAGTAAGATAG
Hairpin Sequence:
5'-CCGG-ACGGCCTGCAGAGTAAGATAG-CTCGAG-CTATCTTACTCTGCAGGCCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGCCTGCAGAGTAAGATAGCTCGAGCTATCTTACTCTGCAGGCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGCCTGCAGAGTAAGATAGCTCGAGCTATCTTACTCTGCAGGCCGT-3'

Other clones with same target sequence:

(none)